pCDH-CMV-mCherry-SFV nsP4

LOCUS       pCDH-CMV-mCherry-SFV nsP4               8994 bp DNA     circular SYN 09-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3219..3260
                     /label=引物 1
     CDS             3240..5081
                     /codon_start=1
                     /label=nsP4
                     /translation="YIFSSDTGSGHLQQKSVRQHNLQCAQLDAVEEEKMYPPKLDTERE
                     KLLLLKMQMHPSEANKSRYQSRKVENMKATVVDRLTSGARLYTGADVGRIPTYAVRYPR
                     PVYSPTVIERFSSPDVAIAACNEYLSRNYPTVASYQITDEYDAYLDMVDGSDSCLDRAT
                     FCPAKLRCYPKHHAYHQPTVRSAVPSPFQNTLQNVLAAATKRNCNVTQMRELPTMDSAV
                     FNVECFKRYACSGEYWEEYAKQPIRITTENITTYVTKLKGPKAAALFAKTHNLVPLQEV
                     PMDRFTVDMKRDVKVTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLNAVLRP
                     NVHTLFDMSAEDFDAIIASHFHPGDPVLETDIASFDKSQDDSLALTGLMILEDLGVDQY
                     LLDLIEAAFGEISSCHLPTGTRFKFGAMMKSGMFLTLFINTVLNITIASRVLEQRLTDS
                     ACAAFIGDDNIVHGVISDKLMAERCASWVNMEVKIIDAVMGEKPPYFCGGFIVFDSVTQ
                     TACRVSDPLKRLFKLGKPLTAEDKQDEDRRRALSDEVSKWFRTGLGAELEVALTSRYEV
                     EGCKSILIAMATLARDIKAFKKLRGPVIHLYGGPRLVR"
     primer_bind     complement(5061..5102)
                     /label=引物 2
     misc_feature    5091..5678
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5562..5573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5751..5984
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6056..6177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6196..6331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6369..6385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6393..6409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6417..6447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6462..6483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6771..7359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7530..8390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8391..8495)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8969..8985
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattct
     3241 atattttctc ctcggacact ggcagcggac atttacaaca aaaatccgtt aggcagcaca
     3301 atctccagtg cgcacaactg gatgcggtcg aggaggagaa aatgtacccg ccaaaattgg
     3361 atactgagag ggagaagctg ttgctgctga aaatgcagat gcacccatcg gaggctaata
     3421 agagtcgata ccagtctcgc aaagtggaga acatgaaagc cacggtggtg gacaggctca
     3481 catcgggggc cagattgtac acgggagcgg acgtaggccg cataccaaca tacgcggttc
     3541 ggtacccccg ccccgtgtac tcccctaccg tgatcgaaag attctcaagc cccgatgtag
     3601 caatcgcagc gtgcaacgaa tacctatcca gaaattaccc aacagtggcg tcgtaccaga
     3661 taacagatga atacgacgca tacttggaca tggttgacgg gtcggatagt tgcttggaca
     3721 gagcgacatt ctgcccggcg aagctccggt gctacccgaa acatcatgcg taccaccagc
     3781 cgactgtacg cagtgccgtc ccgtcaccct ttcagaacac actacagaac gtgctagcgg
     3841 ccgccaccaa gagaaactgc aacgtcacgc aaatgcgaga actacccacc atggactcgg
     3901 cagtgttcaa cgtggagtgc ttcaagcgct atgcctgctc cggagaatat tgggaagaat
     3961 atgctaaaca acctatccgg ataaccactg agaacatcac tacctatgtg accaaattga
     4021 aaggcccgaa agctgctgcc ttgttcgcta agacccacaa cttggttccg ctgcaggagg
     4081 ttcccatgga cagattcacg gtcgacatga aacgagatgt caaagtcact ccagggacga
     4141 aacacacaga ggaaagaccc aaagtccagg taattcaagc agcggagcca ttggcgaccg
     4201 cttacctgtg cggcatccac agggaattag taaggagact aaatgctgtg ttacgcccta
     4261 acgtgcacac attgtttgat atgtcggccg aagactttga cgcgatcatc gcctctcact
     4321 tccacccagg agacccggtt ctagagacgg acattgcatc attcgacaaa agccaggacg
     4381 actccttggc tcttacaggt ttaatgatcc tcgaagatct aggggtggat cagtacctgc
     4441 tggacttgat cgaggcagcc tttggggaaa tatccagctg tcacctacca actggcacgc
     4501 gcttcaagtt cggagctatg atgaaatcgg gcatgtttct gactttgttt attaacactg
     4561 ttttgaacat caccatagca agcagggtac tggagcagag actcactgac tccgcctgtg
     4621 cggccttcat cggcgacgac aacatcgttc acggagtgat ctccgacaag ctgatggcgg
     4681 agaggtgcgc gtcgtgggtc aacatggagg tgaagatcat tgacgctgtc atgggcgaaa
     4741 aacccccata tttttgtggg ggattcatag tttttgacag cgtcacacag accgcctgcc
     4801 gtgtttcaga cccacttaag cgcctgttca agttgggtaa gccgctaaca gctgaagaca
     4861 agcaggacga agacaggcga cgagcactga gtgacgaggt tagcaagtgg ttccggacag
     4921 gcttgggggc cgaactggag gtggcactaa catctaggta tgaggtagag ggctgcaaaa
     4981 gtatcctcat agccatggcc accttggcga gggacattaa ggcgtttaag aaattgagag
     5041 gacctgttat acacctctac ggcggtccta gattggtgcg ttgaggatcc aatcaacctc
     5101 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5161 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5221 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5281 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5341 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5401 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5461 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5521 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5581 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc
     5641 ctcagacgag tcggatctcc ctttggccgc ctccccgccg gtacctttaa gaccaatgac
     5701 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5761 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     5821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6001 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6181 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6301 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     6361 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6421 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7201 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8581 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8641 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8701 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8761 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8821 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8881 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8941 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//